Scientists of the CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, who have analysed more than 2000 genomes of the novel SARS-CoV-2 virus, have found that the clade or genetic group circulating in India has 70% similarity with the major strain around the world. The similarity in viral genome globally means a vaccine or a drug targeting the mutation in A2a clade will work with … Continue reading CSIR scientists find viral strain circulating in India has 70% similarity with major strain around the world

Bone is a highly dynamic organ that undergoes remodeling equally regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. To clarify the regulation of osteoblastogenesis, primary murine osteoblasts are required for an in vitro study. Primary osteoblasts are isolated from neonatal calvariae through digestion with collagenase. However, the number of cells collected from one pup is not sufficient for further in vitro experiments, leading to … Continue reading Endonuclease increases efficiency of osteoblast isolation

In this research scientist study about Cancer cells. They are a major source of enzymes that modify collagen to create a stiff, fibrotic tumor stroma. High collagen lysyl hydroxylase 2 (LH2) expression promotes metastasis and is correlated with shorter survival in lung adenocarcinoma (LUAD) and other tumor types. LH2 hydroxylates lysine (Lys) residues on fibrillar collagen’s amino- and carboxy-terminal telopeptides to create stable collagen cross-links. … Continue reading A collagen glucosyltransferase drives lung adenocarcinoma

mRNA technologies have recently proven clinical efficacy against coronavirus disease 2019 and are among the most promising technologies to address the current pandemic. Here, we show preclinical data for our clinical candidate CVnCoV, a lipid nanoparticle-encapsulated mRNA vaccine that encodes full-length, pre-fusion stabilised severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. In contrast to previously published approaches, CVnCoV is exclusively composed of naturally occurring nucleotides. … Continue reading mRNA-based SARS-CoV-2 vaccine candidate CVnCoV

In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well as for in-vivo cell therapies and tissue engineering approaches. A reliable use of 3D cellular models still requires standardized protocols with well-controlled and … Continue reading Self-organization and culture of Mesenchymal Stem Cell

New Delhi: US pharma giant Johnson & Johnson has said it is in talks with the Modi Johnson has said it is in talks with the Modi government to start clinical trials for its single-shot Covid-19 vaccine in India. The company has informed the Central Drugs Standard Control Organisation (CDSCO), the Indian drug regulator, about its plan to start “bridging clinical trials” soon. The move will help India ramp up the vaccination drive … Continue reading J&J in talks with Modi govt to start clinical trials for its single-shot Covid vaccine in India

Malaria is one of the major parasitic diseases in tropical and subtropical regions which is caused by the genus Plasmodium and transmitted to human beings through the bites of female Anopheles sp. mosquitoes. About 40% of the world’s population lives in malaria-endemic areas. It causes about 1 million deaths per year globally. The situation gets worsened by the widespread development of parasite lines becoming resistant … Continue reading Combating Multi-drug Resistant Malaria Parasite

Postoperative delirium is a common neuro psychiatric syndrome resulting a high postsurgical mortality rate and decline in postdischarge function. Extensive research has been performed on both human and animal delirium-like models due to their clinical significance, focusing on systematic inflammation and consequent neuroinflammation playing a key role in the pathogenesis of postoperative cognitive dysfunctions. Since animal models are widely utilized for pathophysiological study of neuropsychiatric … Continue reading Scopolamine promotes neuroinflammation

The Brain Atlas explores the protein expression in the mammalian brain by visualization and integration of data from three species of mammals: human, pig and mouse. The open-access resource is based on the analysis of nearly 1,900 brain samples covering 27 brain regions. Fig: Genome-wide transcriptomics analysis of anatomically dissected regions in mammalian brains uncovers regional and species-specific expression: multiple regions of the human, pig, and mouse brain … Continue reading Researchers Create Atlas of Protein-Coding Genes in Mammalian Brain

In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting “hot regions,” i.e., amino acid substitutions therein frequently increase the affinities, is desirable for straightforward discovery of valuable mutants. We here report two “designed” site-directed mutagenesis (A and B) targeted the N-terminal 1–10 positions of the VH framework … Continue reading The VH framework region 1 as a target of efficient mutagenesis for generating