Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude … Continue reading High throughput viscoelastic particle focusing

The amyloidoses constitute a group of diseases occurring in humans and animals that are characterized by abnormal deposits of aggregated proteins in organs, affecting their structure and function. In the Abyssinian cat breed, a familial form of renal amyloidosis has been described. In this study, multi-omics analyses were applied and integrated to explore some aspects of the unknown pathogenetic processes in cats. Whole-genome sequences of … Continue reading Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

High-throughput sequencing reveals the complex landscape of small noncoding RNAs (sRNAs). However, it is limited by requiring 5′-monophosphate and 3′-hydroxyl in RNAs for adapter ligation and hindered by methylated nucleosides that interfere with reverse transcription. Here we develop Cap-Clip acid pyrophosphatase (Cap-Clip), T4 polynucleotide kinase (PNK) and AlkB/AlkB(D135S)-facilitated small ncRNA sequencing (CPA-seq) to detect and quantify sRNAs with terminus multiplicities and nucleoside methylations. CPA-seq identified … Continue reading CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini

Expanding the portfolio of products that can be made from lignin will be critical to enabling a viable bio-based economy. Here, we engineer Pseudomonas putida for high-yield production of the tricarboxylic acid cycle-derived building block chemical, itaconic acid, from model aromatic compounds and aromatics derived from lignin. We develop a nitrogen starvation-detecting biosensor for dynamic two-stage bioproduction in which itaconic acid is produced during a non-growth associated … Continue reading Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion

This study is about the development biology i.e.  piriform cortex (PC) is a major cortical processing center for the sense of smell that receives direct inputs from the olfactory bulb. In mice, the PC consists of three neuronal layers, which are populated by cells with distinct developmental origins. One origin of PC neurons is the pool of Dbx1-expressing neural progenitors located in the ventral pallium … Continue reading Specific contribution of neurons from the Dbx1 lineage

In this study the researcher studied about the bacterial species  Streptococcus agalactiae, a commensal urogenital tract bacterium and opportunistic pathogen, can produce new DNA using human urine, according to new research from Griffith University. Fig: Streptococcus agalactiae (round spheres) on the surface of the bladder where they encounter urine to make their DNA.  Dr. Matthew Sullivan and his colleagues from Griffith University found that guaA, a specialized gene … Continue reading Streptococci Can Generate DNA using Human Urine

A team of biologists from the Central Mindanao University has discovered a new endemic species of carnivorous plant in the montane tropical rainforests of Philippines. The newly-discovered species belongs to Nepenthes, a genus of tropical pitcher plants in the monotypic family Nepenthaceae. The genus comprises over 170 species, mostly native to Madagascar, Southeast Asia, and Australia. The greatest diversity occurs on Borneo, Sumatra, and the … Continue reading New Species of Pitcher Plant Discovered in Philippines

Regulation of mRNA translation elongation impacts nascent protein synthesis and integrity and plays a critical role in disease establishment. Here, we investigate features linking regulation of codon-dependent translation elongation to protein expression and homeostasis. Using knockdown models of enzymes that catalyze the mcm5s2 wobble uridine tRNA modification (U34-enzymes), we show that gene codon content is necessary but not sufficient to predict protein fate. While translation … Continue reading Wobble tRNA modification and hydrophilic amino acid

In 1855, the German physician Rudolf Virchow coined the phrase Omnis cellula e cellula — all cells come from cells. In other words, cells arise from the growth and division of existing cells. The genetic information stored in chromosomes is passed on to the next generation during cell division in a highly ordered process called mitosis. Biologists have spent many decades deciphering the molecular choreography … Continue reading Mitochondria are mixed during cell division