A collagen glucosyltransferase drives lung adenocarcinoma

In this research scientist study about Cancer cells. They are a major source of enzymes that modify collagen to create a stiff, fibrotic tumor stroma. High collagen lysyl hydroxylase 2 (LH2) expression promotes metastasis and is correlated with shorter survival in lung adenocarcinoma (LUAD) and other tumor types. LH2 hydroxylates lysine (Lys) residues on fibrillar collagen’s amino- and carboxy-terminal telopeptides to create stable collagen cross-links. … Continue reading A collagen glucosyltransferase drives lung adenocarcinoma

Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

The amyloidoses constitute a group of diseases occurring in humans and animals that are characterized by abnormal deposits of aggregated proteins in organs, affecting their structure and function. In the Abyssinian cat breed, a familial form of renal amyloidosis has been described. In this study, multi-omics analyses were applied and integrated to explore some aspects of the unknown pathogenetic processes in cats. Whole-genome sequences of … Continue reading Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

mRNA-based SARS-CoV-2 vaccine candidate CVnCoV

mRNA technologies have recently proven clinical efficacy against coronavirus disease 2019 and are among the most promising technologies to address the current pandemic. Here, we show preclinical data for our clinical candidate CVnCoV, a lipid nanoparticle-encapsulated mRNA vaccine that encodes full-length, pre-fusion stabilised severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. In contrast to previously published approaches, CVnCoV is exclusively composed of naturally occurring nucleotides. … Continue reading mRNA-based SARS-CoV-2 vaccine candidate CVnCoV

The molecular pH-response mechanism

Plants need to protect themselves from excess light, which causes photo-oxidative damage and lowers the efficiency of photosynthesis. Photosystem II subunit S (PsbS) is a pH sensor protein that plays a crucial role in plant photoprotection by detecting thylakoid lumen acidification in excess light conditions via two lumen-faced glutamates. However, how PsbS is activated under low-pH conditions is unknown. To reveal the molecular response of … Continue reading The molecular pH-response mechanism

CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini

High-throughput sequencing reveals the complex landscape of small noncoding RNAs (sRNAs). However, it is limited by requiring 5′-monophosphate and 3′-hydroxyl in RNAs for adapter ligation and hindered by methylated nucleosides that interfere with reverse transcription. Here we develop Cap-Clip acid pyrophosphatase (Cap-Clip), T4 polynucleotide kinase (PNK) and AlkB/AlkB(D135S)-facilitated small ncRNA sequencing (CPA-seq) to detect and quantify sRNAs with terminus multiplicities and nucleoside methylations. CPA-seq identified … Continue reading CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini

Self-organization and culture of Mesenchymal Stem Cell

In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well as for in-vivo cell therapies and tissue engineering approaches. A reliable use of 3D cellular models still requires standardized protocols with well-controlled and … Continue reading Self-organization and culture of Mesenchymal Stem Cell

Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion

Expanding the portfolio of products that can be made from lignin will be critical to enabling a viable bio-based economy. Here, we engineer Pseudomonas putida for high-yield production of the tricarboxylic acid cycle-derived building block chemical, itaconic acid, from model aromatic compounds and aromatics derived from lignin. We develop a nitrogen starvation-detecting biosensor for dynamic two-stage bioproduction in which itaconic acid is produced during a non-growth associated … Continue reading Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion

J&J in talks with Modi govt to start clinical trials for its single-shot Covid vaccine in India

New Delhi: US pharma giant Johnson & Johnson has said it is in talks with the Modi Johnson has said it is in talks with the Modi government to start clinical trials for its single-shot Covid-19 vaccine in India. The company has informed the Central Drugs Standard Control Organisation (CDSCO), the Indian drug regulator, about its plan to start “bridging clinical trials” soon. The move will help India ramp up the vaccination drive … Continue reading J&J in talks with Modi govt to start clinical trials for its single-shot Covid vaccine in India

Search Indigenous System for Assessment and Relearning of Postural Balance

Postural stability is achieved by maintaining an upright body alignment against gravitational force and preserving the equilibrium of the Centre of Pressure (CoP) in an individual’s base of support. Successful postural control requires contribution from a complex sensory system comprising visual, somatosensory and vestibular modalities as well as motor control systems.  Due to recent technological advances, a growing trend in balance assessments has become the … Continue reading Search Indigenous System for Assessment and Relearning of Postural Balance

Combating Multi-drug Resistant Malaria Parasite

Malaria is one of the major parasitic diseases in tropical and subtropical regions which is caused by the genus Plasmodium and transmitted to human beings through the bites of female Anopheles sp. mosquitoes. About 40% of the world’s population lives in malaria-endemic areas. It causes about 1 million deaths per year globally. The situation gets worsened by the widespread development of parasite lines becoming resistant … Continue reading Combating Multi-drug Resistant Malaria Parasite