Resolving cell state in iPSC-derived human neural samples

Human induced pluripotent stem cell-derived (iPSC) neural cultures offer clinically relevant models of human diseases, including Amyotrophic Lateral Sclerosis, Alzheimer’s, and Autism Spectrum Disorder. In situ characterization of the spatial-temporal evolution of cell state in 3D culture and subsequent 2D dissociated culture models based on protein expression levels and localizations is essential to understanding neural cell differentiation, disease state phenotypes, and sample-to-sample variability. Here, we apply PRobe-based Imaging for SequentialMultiplexing (PRISM) to facilitate multiplexed imaging with facile, rapid exchange of imaging probes to analyze iPSC-derived cortical and motor neuron cultures that are relevant to psychiatric and neurodegenerative disease models, using over ten protein targets.

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Fig: Generating stem cell derived disease models and complex neuronal cultures based on patient iPSCs requires careful and extensive validation.

Our approach permits analysis of cell differentiation, cell composition, and functional marker expression in complex stem-cell derived neural cultures. Furthermore, our approach is amenable to automation, offering in principle the ability to scale-up to dozens of protein targets and samples.

Tomov, M.L., O’Neil, A., Abbasi, H.S. et al. Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging. Commun Biol 4, 786 (2021). https://doi.org/10.1038/s42003-021-02276-x

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