A Ludwig Cancer Research study has discovered how to revive a powerful but functionally inert subset of anti-cancer immune cells that are often found within tumors for cancer therapy. Led by Ludwig Lausanne’s Ping-Chih Ho and Li Tang of the École Polytechnique Fédérale de Lausanne, the study describes how an immune factor known as interleukin-10 orchestrates the functional revival of “terminally exhausted” tumor-infiltrating T lymphocytes (TILs), which have so far proved impervious to stimulation by immunotherapies. It also demonstrates that the factor, when applied in combination with cell therapies, can eliminate tumors in mouse models of melanoma and colon cancer. The findings are reported in the current issue of Nature Immunology. The first time, terminally exhausted TILs can be directly rejuvenated so that their potent anti-cancer activity is restored, and that this rejuvenation is accomplished through the metabolic reprogramming of the cells induced by interleukin-10.
Deprived of oxygen and vital nutrients within tumors, the TILs most capable of killing cancer cells are typically pushed into a stubbornly sluggish state known as exhaustion. Recent research has identified two distinct types of exhausted TILs. One, known as “progenitor exhausted” TILs, can recognize cancer cells with nominal efficiency and proliferate in response to the immunotherapy PD-1 blockade. But it is their descendants, “terminally exhausted” TILs, that are best equipped to detect and destroy cancer cells. They are, however, functionally disabled, prone to self-destruction, and utterly incapable of proliferation.
Three lines of evidence prompted the current study. First, Ho and his team recently showed that terminally exhausted TILs could be functionally revived by restoring the health of their mitochondria—the bean-shaped organelles in cells that generate energy and help regulate metabolism. Second, IL-10 is known to stimulate anti-cancer immune responses and has been tested as a therapy for lung cancer in an early phase clinical trial, albeit with mixed results. Finally, IL-10 was recently found to restore mitochondrial fitness and reprogram the metabolism of immune cells known as macrophages.
To find out, they added an engineered, long-lived version of IL-10 (IL-10/Fc) to adoptive cell therapy (ACT)—the infusion of tumor-targeting T cells to treat cancer—and tested the combination in a mouse model of melanoma. The treatment boosted the number and functionality of terminally exhausted TILs and led to tumor regression and cures in 90% of treated mice, compared to limited regressions with IL-10/Fc alone and none with ACT alone. Notably, 80% of surviving mice developed an immune memory for cancer, spontaneously rejecting the same tumors implanted two months after therapy. The researchers also tested IL-10/Fc on CAR-T cells, which are engineered to target cancer cells bearing specific molecular markers. IL-10/Fc treated CAR-T cells induced complete cures of roughly 90% of mice implanted with colon cancer tumors. IL-10/Fc specifically acts on terminally exhausted TILs, not on progenitor exhausted TILs. They also showed it reprograms the metabolism of terminally exhausted TILs—beginning with the process they employ to extract energy from nutrients. That, in turn, leads to sweeping changes in their gene expression programs, which drives their functional re-activation and proliferation. IL-10/Fc had the same effect on human TILs, and on CAR-T cells as well.
The researchers are now investigating the precise mechanisms by which metabolic reprogramming alters gene expression patterns in terminally exhausted TILs. They are also conducting preclinical studies to advance the application of their discoveries to human cancer therapy.
Yugang Guo et al, Metabolic reprogramming of terminally exhausted CD8+ T cells by IL-10 enhances anti-tumor immunity, Nature Immunology (2021). DOI: 10.1038/s41590-021-00940-2