iTRAQ-based proteome profiling revealed the role of Phytochrome A

In this study researcher selected  plants, during growth and development, photoreceptors monitor fluctuations in their environment and adjust their metabolism as a strategy of surveillance. Phytochromes (Phys) play an essential role in plant growth and development, from germination to fruit development. FR-light (FR) insensitive mutant (fri) carries a recessive mutation in Phytochrome A and is characterized by the failure to de-etiolate in continuous FR. Here we used iTRAQ-based quantitative proteomics along with metabolomics to unravel the role of Phytochrome A in regulating central metabolism in tomato seedlings grown under FR. Our results indicate that Phytochrome A has a predominant role in FR-mediated establishment of the mature seedling proteome. Further, we observed temporal regulation in the expression of several of the late response proteins associated with central metabolism. The proteomics investigations identified a decreased abundance of enzymes involved in photosynthesis and carbon fixation in the mutant.

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Fig: Quantitative proteomics and metabolite profiling of tomato seedlings to understand the role of PhyA in FR light-mediated photomorphogenesis. (A) Schematic illustration of the overall experimental strategy used in the discovery-phase global quantitative proteomics and metabolomics. (B) Venn diagram showing the unique and overlapping proteins identified in specific light treatment at two different time points.

Profound accumulation of storage proteins in the mutant ascertained the possible conversion of sugars into storage material instead of being used or the retention of an earlier profile associated with the mature embryo. The enhanced accumulation of organic sugars in the seedlings indicates the absence of photomorphogenesis in the mutant.

Thomas, S., Kumar, R., Sharma, K. et al. iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling. Sci Rep 11, 7540 (2021). https://doi.org/10.1038/s41598-021-87208-9

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