CSIR scientists find viral strain circulating in India has 70% similarity with major strain around the world

Scientists of the CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, who have analysed more than 2000 genomes of the novel SARS-CoV-2 virus, have found that the clade or genetic group circulating in India has 70% similarity with the major strain around the world. The similarity in viral genome globally means a vaccine or a drug targeting the mutation in A2a clade will work with … Continue reading CSIR scientists find viral strain circulating in India has 70% similarity with major strain around the world

Ground Improvement Technique to Mitigate Liquefaction Hazards for Safe Building Construction

Soil liquefaction and its associated ground failures during an earthquake are major potential hazards. The important aspect of geotechnical earthquake engineering is to mitigate liquefaction and its associated effects for assuring the safety of foundations. There are different ground improvement techniques available for improving the liquefaction resistance of sand deposits. Ground improvement techniques such as sand compaction piles, stone columns, encased stone columns and prefabricated … Continue reading Ground Improvement Technique to Mitigate Liquefaction Hazards for Safe Building Construction

High throughput viscoelastic particle focusing

Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude … Continue reading High throughput viscoelastic particle focusing

Endonuclease increases efficiency of osteoblast isolation

Bone is a highly dynamic organ that undergoes remodeling equally regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. To clarify the regulation of osteoblastogenesis, primary murine osteoblasts are required for an in vitro study. Primary osteoblasts are isolated from neonatal calvariae through digestion with collagenase. However, the number of cells collected from one pup is not sufficient for further in vitro experiments, leading to … Continue reading Endonuclease increases efficiency of osteoblast isolation

Antifreeze protein from Ammopiptanthus nanus

In this research resercher discuss about the Temperature stress restricts in plant growth and development. Antifreeze protein (AFP) can improve plants antifreeze ability. In our previous study, the AnAFP gene cloned from Ammopiptanthus nanus was confirmed to be an excellent candidate enhancing plant cold resistance. But, AnAFP protein shared similar structures with KnS type dehydrins including K, N and S domains except ice crystal binding … Continue reading Antifreeze protein from Ammopiptanthus nanus

A collagen glucosyltransferase drives lung adenocarcinoma

In this research scientist study about Cancer cells. They are a major source of enzymes that modify collagen to create a stiff, fibrotic tumor stroma. High collagen lysyl hydroxylase 2 (LH2) expression promotes metastasis and is correlated with shorter survival in lung adenocarcinoma (LUAD) and other tumor types. LH2 hydroxylates lysine (Lys) residues on fibrillar collagen’s amino- and carboxy-terminal telopeptides to create stable collagen cross-links. … Continue reading A collagen glucosyltransferase drives lung adenocarcinoma

Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

The amyloidoses constitute a group of diseases occurring in humans and animals that are characterized by abnormal deposits of aggregated proteins in organs, affecting their structure and function. In the Abyssinian cat breed, a familial form of renal amyloidosis has been described. In this study, multi-omics analyses were applied and integrated to explore some aspects of the unknown pathogenetic processes in cats. Whole-genome sequences of … Continue reading Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits

mRNA-based SARS-CoV-2 vaccine candidate CVnCoV

mRNA technologies have recently proven clinical efficacy against coronavirus disease 2019 and are among the most promising technologies to address the current pandemic. Here, we show preclinical data for our clinical candidate CVnCoV, a lipid nanoparticle-encapsulated mRNA vaccine that encodes full-length, pre-fusion stabilised severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. In contrast to previously published approaches, CVnCoV is exclusively composed of naturally occurring nucleotides. … Continue reading mRNA-based SARS-CoV-2 vaccine candidate CVnCoV

The molecular pH-response mechanism

Plants need to protect themselves from excess light, which causes photo-oxidative damage and lowers the efficiency of photosynthesis. Photosystem II subunit S (PsbS) is a pH sensor protein that plays a crucial role in plant photoprotection by detecting thylakoid lumen acidification in excess light conditions via two lumen-faced glutamates. However, how PsbS is activated under low-pH conditions is unknown. To reveal the molecular response of … Continue reading The molecular pH-response mechanism

CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini

High-throughput sequencing reveals the complex landscape of small noncoding RNAs (sRNAs). However, it is limited by requiring 5′-monophosphate and 3′-hydroxyl in RNAs for adapter ligation and hindered by methylated nucleosides that interfere with reverse transcription. Here we develop Cap-Clip acid pyrophosphatase (Cap-Clip), T4 polynucleotide kinase (PNK) and AlkB/AlkB(D135S)-facilitated small ncRNA sequencing (CPA-seq) to detect and quantify sRNAs with terminus multiplicities and nucleoside methylations. CPA-seq identified … Continue reading CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini