A prostate-specific membrane antigen activated molecular rotor

Surgery is an efficient way to treat localized prostate cancer (PCa), however, it is challenging to demarcate rapidly and accurately the tumor boundary intraoperatively, as existing tumor detection methods are seldom performed in real-time. To overcome those limitations, we develop a fluorescent molecular rotor that specifically targets the prostate-specific membrane antigen (PSMA), an established marker for PCa. The probes have picomolar affinity (IC50 = 63-118 pM) for … Continue reading A prostate-specific membrane antigen activated molecular rotor

A scalable workflow to characterize the human exposome

Complementing the genome with an understanding of the human exposome is an important challenge for contemporary science and technology. Tens of thousands of chemicals are used in commerce, yet cost for targeted environmental chemical analysis limits surveillance to a few hundred known hazards. To overcome limitations which prevent scaling to thousands of chemicals, we develop a single-step express liquid extraction and gas chromatography high-resolution mass … Continue reading A scalable workflow to characterize the human exposome

Molecular basis for PICS-mediated piRNA biogenesis and cell division

By incorporating two mutually exclusive factors, PID-1 and TOST-1, C. elegans PICS complex plays important roles in piRNA biogenesis, chromosome segregation and cell division. We firstly map the interaction network between PICS subunits, then uncover the mechanisms underlying the interactions between PICS subunits by solving several complex structures, including those of TOFU-6/PICS-1, ERH-2/PICS-1, and ERH-2/TOST-1. Our biochemical experiment also demonstrates that PICS exists as an octamer consisting … Continue reading Molecular basis for PICS-mediated piRNA biogenesis and cell division

Influence of storms on the emission of pollutants from sewage into waters

Fig: Arrangement of rain gauges and storm overflows on the sewage network in the area of the considered catchment. OS-1 ÷ 3—overflow structures; 1–6—rain gauges; STP—sewage treatment plant. During heavy precipitation, chemical and biological pollutants from urban and agricultural areas enter the waters from storm overflows as a result of infiltration and inflow, as well as via uncontrolled outflows from water treatment plants. Infiltration and inflow of … Continue reading Influence of storms on the emission of pollutants from sewage into waters

Liquid condensation of reprogramming factor KLF4 with DNA

FIG: KLF4 forms a condensed liquid phase in HEK 293T cells and BJ fibroblasts Expression of a few master transcription factors can reprogram the epigenetic landscape and three-dimensional chromatin topology of differentiated cells and achieve pluripotency. During reprogramming, thousands of long-range chromatin contacts are altered, and changes in promoter association with enhancers dramatically influence transcription. Molecular participants at these sites have been identified, but how this … Continue reading Liquid condensation of reprogramming factor KLF4 with DNA

Structural basis of human PDZD8–Rab7

Fig: The overall structure of the human PDZD8 CC–Rab7 complex. (A) Schematic representation of the domain structures of human PDZD8 and Rab7. The domains with the structures determined in this study are indicated with blue arrows. (B) 2.1 Å 2F0–Fc electron density maps with the final model superimposed. The Rab7 structure is shown in green and the PDZD8 CC in yellow. (C) Overall structure of the PDZD8 … Continue reading Structural basis of human PDZD8–Rab7

CSIR-CCMB Open Days 2021

CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, will be organising CCMB Open Days 2021 from 23 to 26 September 2021. CCMB scientists will meet and discuss the different questions they address in life sciences. There will be discussions on how to be a scientist, how to find the right information and cool competitions. Registration link for Lab sessions:https://us06web.zoom.us/webinar/register/WN_bujZmgwgRAK15bsXZqDrMw Registration link for Open Day Competitions:https://docs.google.com/forms/d/e/1FAIpQLSdGttUcpGrZYMkoXvFpIQx-XHiouy5kxMPrO0yPJsIbT2oUMA/viewform Continue reading CSIR-CCMB Open Days 2021

First-ever Monk Fruit Cultivation in India by CSIR-IHBT

Intake of added cane sugars can lead to lifestyle-associated diseases such as insulin resistance, type-2 diabetes, liver problems, metabolic syndrome, heart disease, etc. As per a World Health Organization (WHO) report, presently about 422 million people are diabetic worldwide. Several synthetic sweeteners of low calorific value have recently been launched in the market by the pharmaceutical and food industries. However, their possible health issues generally … Continue reading First-ever Monk Fruit Cultivation in India by CSIR-IHBT

Decreased Coloured Dissolved Organic Matter Build-up and Nutrients

A team of researchers at CSIR-National Institute of Oceanography (CSIR-NIO), Goa, studied the variations of Coloured Dissolved Organic Matter (CDOM) in the Mandovi Estuary, Goa — “During Spring Inter-Monsoon: A Comparison with COVID-19 Outbreak Imposed Lockdown Period”. One of the chemical parameters of importance is the Dissolved Organic Matter (DOM) in the water, which is the largest reservoir of organic carbon in the aquatic environment. … Continue reading Decreased Coloured Dissolved Organic Matter Build-up and Nutrients

Epistasis shapes the fitness landscape of an allosteric specificity switch

Epistasis is a major determinant in the emergence of novel protein function. In allosteric proteins, direct interactions between inducer-binding mutations propagate through the allosteric network, manifesting as epistasis at the level of biological function. Elucidating this relationship between local interactions and their global effects is essential to understanding evolution of allosteric proteins. We integrate computational design, structural and biophysical analysis to characterize the emergence of … Continue reading Epistasis shapes the fitness landscape of an allosteric specificity switch